Pubblicazioni

Autori:

Vettori, Marco; Dima, Francesco; Henry, Brandon Michael; Carpenè, Giovanni; Gelati, Matteo; Celegon, Giovanni; Salvagno, Gian Luca; Lippi, Giuseppe

Titolo:

Effects of Different Types of Recombinant SARS-CoV-2 Spike Protein on Circulating Monocytes’ Structure

Anno:

2023

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN Rivista:

1422-0067

N° Volume:

24

Numero o Fascicolo:

11

Intervallo pagine:

9373-9373

Parole chiave:

SARS-CoV-2; COVID-19; monocyte; spike protein; morphology

Breve descrizione dei contenuti:

This study investigated the biological effects on circulating monocytes after challenge with SARS-CoV-2 recombinant spike protein. Whole blood collected from seven ostensibly healthy healthcare workers was incubated for 15 min with 2 and 20 ng/mL final concentration of recombinant spike protein of Ancestral, Alpha, Delta, and Omicron variants. Samples were analyzed with Sysmex XN and DI-60 analyzers. Cellular complexity (i.e., the presence of granules, vacuoles and other cytoplasmic inclusions) increased in all samples challenged with the recombinant spike protein of the Ancestral, Alpha, and Delta variants, but not in those containing Omicron. The cellular content of nucleic acids was constantly decreased in most samples, achieving statistical significance in those containing 20 ng/mL of Alpha and Delta recombinant spike proteins. The heterogeneity of monocyte volumes significantly increased in all samples, achieving statistical significance in those containing 20 ng/mL of recombinant spike protein of the Ancestral, Alpha and Delta variants. The monocyte morphological abnormalities after spike protein challenge included dysmorphia, granulation, intense vacuolization, platelet phagocytosis, development of aberrant nuclei, and cytoplasmic extrusions. The SARS-CoV-2 spike protein triggers important monocyte morphological abnormalities, more evident in cells challenged with recombinant spike protein of the more clinically severe Alpha and Delta variants.

Pagina Web:

https://www.mdpi.com/1422-0067/24/11/9373

Id prodotto:

134014

Handle IRIS:

11562/1094972

ultima modifica:

24 agosto 2023

Citazione bibliografica:

Vettori, Marco; Dima, Francesco; Henry, Brandon Michael; Carpenè, Giovanni; Gelati, Matteo; Celegon, Giovanni; Salvagno, Gian Luca; Lippi, Giuseppe, Effects of Different Types of Recombinant SARS-CoV-2 Spike Protein on Circulating Monocytes’ Structure «INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES» , vol. 24 , n. 11 , 2023 , pp. 9373-9373

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