We will exploit some potential advantages of Fourier Transform InfraRed absorbance microspectroscoy (microFT-IR) to identify biochemical and morphological effects of drugs by SR IR microanalysis on single cells. The goal is to start developing a reference library of validated FTIR spectra via Synchrotron Radiation IR microprobe for the future application of microFT-IR analysis as a drug screening test and for clinical follow-up of patients with leukaemia. We expect to acquire the maximal number of representative spectra with acceptable signal-to-noise ratio (S/N) values for statistics significance in samples. In parallel, complementary biochemical information achieved in samples will be associated with spectral information allowing to cross-validate
reference FT-IR absorbance spectra of functional and structural cellular events induced by drugs in cancer cell models.
