Publications

Authors:

Sorio, Claudio; Bellisola, Giuseppe

Title:

InfraRed Spectroscopy and Microscopy in Cancer Research

Year:

2012

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

AMERICAN JOURNAL OF CANCER RESEARCH

ISSN of journal:

2156-6976

N° Volume:

2

Number or Folder:

1

Page numbers:

1-21

Keyword:

Molecular vibrations; vibrational spectroscopy; infrared radiation; synchrotron radiation; infrared microspectroscopy; mid-infrared absorbance spectroscopy; cancer biomarker; cancer diagnosis; pre-clinical drug screening; unsupervised multivariate analysis

Short description of contents:

Since the middle of 20th century infrared (IR) spectroscopy coupled to microscopy (IR microspectroscopy) has been recognized as a non destructive, label free, highly sensitive and specific analytical method with many potential useful applications in different fields of biomedical research and in particular cancer research and diagnosis. Although many technological improvements have been made to facilitate biomedical applications of this powerful analytical technique, it has not yet properly come into the scientific background of many potential end users. Therefore, to achieve those fundamental objectives an interdisciplinary approach is needed with basic scientists, spectroscopists, biologists and clinicians who must effectively communicate and understand each other’s requirements and challenges. In this review we aim at illustrating some principles of Fourier transform (FT) Infrared (IR) vibrational spectroscopy and microscopy (microFT-IR) as a useful method to interrogate molecules in specimen by mid-IR radiation. Penetrating into basics of molecular vibrations might help us to understand whether, when and how complementary information obtained by microFT-IR could become useful in our research and/or diagnostic activities. MicroFT-IR techniques allowing to acquire information about the molecular composition and structure of a sample within a micrometric scale in a matter of seconds will be illustrated as well as some limitations will be discussed. How biochemical, structural, and dynamical information about the systems can be obtained by bench top microFTIR instrumentation will be also presented together with some methods to treat and interpret IR spectral data and applicative examples. The mid-IR absorbance spectrum is one of the most information-rich and concise way to represent the whole “…omics” of a cell and, as such, fits all the characteristics for the development of a clinically useful biomarker.

Web page:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236568/?tool=pubmed

Product ID:

64574

Handle IRIS:

11562/367993

Deposited On:

January 4, 2012

Last Modified:

November 15, 2022

Bibliographic citation:

Sorio, Claudio; Bellisola, Giuseppe, InfraRed Spectroscopy and Microscopy in Cancer Research «AMERICAN JOURNAL OF CANCER RESEARCH» , vol. 2 , n. 1 , 2012 , pp. 1-21

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