Publications

Authors:

Vettori, Marco; Dima, Francesco; Henry, Brandon Michael; Carpenè, Giovanni; Gelati, Matteo; Celegon, Giovanni; Salvagno, Gian Luca; Lippi, Giuseppe

Title:

Effects of Different Types of Recombinant SARS-CoV-2 Spike Protein on Circulating Monocytes’ Structure

Year:

2023

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN of journal:

1422-0067

N° Volume:

24

Number or Folder:

11

Page numbers:

9373-9373

Keyword:

SARS-CoV-2; COVID-19; monocyte; spike protein; morphology

Short description of contents:

This study investigated the biological effects on circulating monocytes after challenge with SARS-CoV-2 recombinant spike protein. Whole blood collected from seven ostensibly healthy healthcare workers was incubated for 15 min with 2 and 20 ng/mL final concentration of recombinant spike protein of Ancestral, Alpha, Delta, and Omicron variants. Samples were analyzed with Sysmex XN and DI-60 analyzers. Cellular complexity (i.e., the presence of granules, vacuoles and other cytoplasmic inclusions) increased in all samples challenged with the recombinant spike protein of the Ancestral, Alpha, and Delta variants, but not in those containing Omicron. The cellular content of nucleic acids was constantly decreased in most samples, achieving statistical significance in those containing 20 ng/mL of Alpha and Delta recombinant spike proteins. The heterogeneity of monocyte volumes significantly increased in all samples, achieving statistical significance in those containing 20 ng/mL of recombinant spike protein of the Ancestral, Alpha and Delta variants. The monocyte morphological abnormalities after spike protein challenge included dysmorphia, granulation, intense vacuolization, platelet phagocytosis, development of aberrant nuclei, and cytoplasmic extrusions. The SARS-CoV-2 spike protein triggers important monocyte morphological abnormalities, more evident in cells challenged with recombinant spike protein of the more clinically severe Alpha and Delta variants.

Web page:

https://www.mdpi.com/1422-0067/24/11/9373

Product ID:

134014

Handle IRIS:

11562/1094972

Last Modified:

August 24, 2023

Bibliographic citation:

Vettori, Marco; Dima, Francesco; Henry, Brandon Michael; Carpenè, Giovanni; Gelati, Matteo; Celegon, Giovanni; Salvagno, Gian Luca; Lippi, Giuseppe, Effects of Different Types of Recombinant SARS-CoV-2 Spike Protein on Circulating Monocytes’ Structure «INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES» , vol. 24 , n. 11 , 2023 , pp. 9373-9373

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo