Publications

Authors:

Salvagno, Gian Luca; Henry, Brandon M; De Nitto, Simone; Pighi, Laura; Lippi, Giuseppe

Title:

Correlation Between Time to Positive Result of SARS-CoV-2 Rapid Antigen Self-Test and Viral Antigen Concentration

Year:

2022

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

No

Name of journal:

EJIFCC

ISSN of journal:

1650-3414

N° Volume:

33

Number or Folder:

4

Page numbers:

309-316

Keyword:

COVID-19, SARS-CoV-2, diagnosis, immunoassay, laboratory medicine

Short description of contents:

Background: This study was planned to investigate how the positivization time of a SARS-CoV-2 rapid antigen self-test may correlate with SARS-CoV-2 nucleocapsid (N) antigen concentration measured with a quantitative laboratory-based immunoassay. Methods: Paired nasopharyngeal (healthcare-collected) and nasal (self-collected) samples were taken from patients undergoing routine SARS-CoV-2 testing. The concentration of SARS-CoV-2 antigen nucleocapsid (N) was assayed with Liaison SARS-CoV-2 Antigen test, whilst the time of positivization of COVID-VIRO ALL rapid diagnostic test (RDT) was concomitantly measured and then compared SARS-CoV-2 viral load measured with Liaison SARS-CoV-2 Antigen test and expressed as Median Tissue Culture Infectious Dose (TCID50)/mL. Results: The study sample consisted of 32 paired specimens which tested positive with COVID-VIRO ALL IN RDT and had SARS-CoV-2 N protein concentration measured with Liaison SARS-CoV-2 Antigen test. A highly significant correlation was found between SARS-CoV-2 viral antigen concentration and RDT positivization time (r=-0.64; 95%CI, -0.81 to -0.38; p<0.001). At the >1500 TCID50/mL threshold of the Liaison SARS-CoV-2 Antigen test, the positivization time of the COVID-VIRO ALL IN RDT displayed high accuracy (93.7%). A positivization time <42 sec enabled to identify patients with high SARS-CoV-2 antigen concentration (i.e., >1500 TCID50/mL) with 91.3% negative and 100% positive predictive values. Conclusion: Self-testing using COVID-VIRO ALL IN RDT could be reliably used for garnering valuable information on the actual SARS-CoV-2 viral antigen concentration in respiratory samples.

Web page:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768622/

Product ID:

131504

Handle IRIS:

11562/1081989

Last Modified:

September 23, 2023

Bibliographic citation:

Salvagno, Gian Luca; Henry, Brandon M; De Nitto, Simone; Pighi, Laura; Lippi, Giuseppe, Correlation Between Time to Positive Result of SARS-CoV-2 Rapid Antigen Self-Test and Viral Antigen Concentration «EJIFCC» , vol. 33 , n. 4 , 2022 , pp. 309-316

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